abstract：:Human endothelial cells have been shown to be distinctive from other IL-1 responsive cells (e.g. murine thymocytes), in the equal units of murine and human IL-1 do not have the same endothelial cell stimulatory effect, as measured by increased lymphocyte adherence. In this paper, the cell surface IL-1 receptors of hum...

journal_title：Molecular immunology

authors： Thieme TR,Wagner CR

更新日期：1989-03-01 00:00:00

abstract：:The carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus) was investigated by quantitative precipitin analysis using blood group A, B, H, Le and I substances and by precipitation inhibition with various mono- and oligosaccharides. The lectin precipitated best with A1 subst...

journal_title：Molecular immunology

authors： Matsuda T,Kabat EA,Surolia A

更新日期：1989-02-01 00:00:00

abstract：:Using the non-crossreactive mAb MRC-OX3 and MRC-OX6, two serologically distinct RT1.B-specific (I-A equivalent) alpha, beta heterodimers have previously been described by us as residing at the cell surface of LEW rat spleen cells. The two-chain elements were suggested to represent stable conformation isomers, diverged...

journal_title：Molecular immunology

authors： Henkes W,Reske K

更新日期：1989-02-01 00:00:00

abstract：:Peptide fragments of pertussis toxin subunit 1 (PT-S1) have been synthesized in order to investigate their antigenic and immunogenic activity, and to evaluate their possible use as components of a new vaccine. Two peptides (sequence 73-82, EAERAGRGTG and sequence 107-116, YVDTYGDNAG) were selected for their predictabl...

journal_title：Molecular immunology

authors： Presentini R,Perin F,Ancilli G,Nucci D,Bartoloni A,Rappuoli R,Antoni G

更新日期：1989-01-01 00:00:00

abstract：:The general thinking about the phylogenic distribution of vertebrate Ig classes is that fish and urodele amphibians are only able to synthesize polymeric IgM-like molecules and that the emergence of a new class of LMW Ig occurs for the first time in anouran species. Following immunization of the Mexican axolotl (Ambys...

journal_title：Molecular immunology

authors： Fellah JS,Charlemagne J

更新日期：1988-12-01 00:00:00

abstract：:The B1 molecule (CD20) is a phosphoprotein expressed only by B-lymphocytes. In this study, analysis of B1 immunoprecipitated from surface iodinated B-cell lines and B-lymphocytes has shown that there are several expressed forms of B1. A predominant species of Mr 33,000 represents 75-80% of the iodinated cell surface B...

journal_title：Molecular immunology

authors： Tedder TF,McIntyre G,Schlossman SF

更新日期：1988-12-01 00:00:00

abstract：:When 5-fluorouracil (5-FU) resistant bone marrow (BM) cells are depleted of B-cells and then cultured in insert chambers [separated from a layer of adherent BM (aBM) cells by a nucleopore membrane], no mature, lipopolysaccharide (LPS) reactive B-cells are formed. Factors acting on B-cell precursors are not produced un...

journal_title：Molecular immunology

authors： Gisler RH,Schlienger C,Söderberg A,Ledermann F,Lambris JD

更新日期：1988-11-01 00:00:00

abstract：:A quantitative analysis of the frequencies of autoantibody producing B-cells has been undertaken by producing and analyzing random hybridoma collections generated in fusions with activated B-cells. Activated B-cells were derived from mice injected with LPS and SRBC and normal mice. They were compared to those derived ...

journal_title：Molecular immunology

authors： Rolink AG,Thalmann P,Berger C,Radaszkiewicz T,Melchers F

更新日期：1988-11-01 00:00:00

abstract：:Stimulation of cells of the rat basophilic leukemia line RBL-2H3, which are used as a model in biochemical studies of mast cells, by antigen or by the calcium ionophore ionomycin, are known to cause secretion of mediators of inflammation. These stimuli have now been found to cause a decrease in the cells' cytosolic pH...

journal_title：Molecular immunology

authors： Gertler R,Pecht I

更新日期：1988-11-01 00:00:00

abstract：:Two anti-phenyloxazolone (phOx3) and one anti-GAT MAbs from C57BL mice are shown to be coded by VH gene 186.2. This gene has been found earlier to code for several anti-NP (NNP) antibodies (Bothwell et al., 1981) and anti-GT antibodies (Rocca-Serra et al., 1983; Carmack and Pincus, 1986). The L chain partner of the VH...

journal_title：Molecular immunology

authors： Kaartinen M,Rocca-Serra J,Mäkelä O

更新日期：1988-09-01 00:00:00

abstract：:T-lymphocyte proliferative responses were determined with peripheral blood lymphocytes (PBL), peritoneal exudate lymphocytes (PEL) and lymph node lymphocytes (LNL) of guinea pigs immunized with horse cytochrome c (HCytc). The proliferative response of PBL activated by peptide 81-104 from animals immunized with HCytc w...

journal_title：Molecular immunology

authors： Wang KM,Reichlin M

更新日期：1988-08-01 00:00:00

abstract：:Syngeneic polyclonal antibodies were elicited to an affinity labeled high affinity (2-3 X 10(10) M-1) anti-fluorescein murine IgG2a monoclonal antibody. Hyperimmune ascites fluid was tested for reactivity with homologous liganded, affinity labeled and non-liganded Fab fragments derived from the high affinity antibody....

journal_title：Molecular immunology

authors： Voss EW Jr,Miklasz SD,Petrossian A,Dombrink-Kurtzman MA

更新日期：1988-08-01 00:00:00

abstract：:Comparative interactions of purified rabbit C1q with 18-2-3, a high affinity (2-3 X 10(10) M-1) anti-fluorescein (anti-F1) murine monoclonal IgM antibody (pentamer) and constitutive monomeric subunits (IgMs) were studied. Using a solid phase radioimmunoassay (SPRIA), based on immobilized polyvalent antigen, it was sho...

journal_title：Molecular immunology

authors： Swanson SM,Dombrink-Kurtzman MA,Voss EW Jr

更新日期：1988-06-01 00:00:00

abstract：:Amino acid sequences encoded by exon 8 of the H-2K and H-2D/L genes appear to be locus specific. The majority of H-2Kb molecules contain 10 amino acids that are derived from exon 8. In contrast, the H-2Db, -Dd and -Ld molecules contain only one amino acid which is encoded by exon 8, even though the genetic information...

journal_title：Molecular immunology

authors： Maloy WL,Lew AM,Anderson RW,Coligan JE

更新日期：1988-05-01 00:00:00

abstract：:In contrast to normal B lymphocytes, splenic B cells from autoimmune NZB mice express on their surface greater amounts of IgM than IgD. We have found that the predominant mu chain species present on these cells is derived from pentameric secretory IgM which can be radioiodinated during transient association with the c...

journal_title：Molecular immunology

authors： Yuan D,Goldings EA

更新日期：1988-04-01 00:00:00

abstract：:The fifth component of human complement (C5), under physiological salt conditions, bound firmly to phenyl-Sepharose. This indicated the presence of hydrophobic sites on C5. These sites may play an important role in C5 haemolytic activity since this activity was inhibited by chaotropic ions (Mg2+, SCN-, I-) at relative...

journal_title：Molecular immunology

authors： Al Salihi A,Ripoche J,Fontaine M

更新日期：1988-04-01 00:00:00

abstract：:Two monoclonal antibodies, HH8 and HH9, have been established after immunization of mice with galactosyl-A glycolipid antigen having the terminal structure, Gal beta 1----3GalNAc alpha 1----3[Fuc alpha 1----2]Gal beta 1----R, which is the precursor for type 3 chain A (repetitive A) and type 3 chain H (A-associated H)....

journal_title：Molecular immunology

authors： Clausen H,Stroud M,Parker J,Springer G,Hakomori S

更新日期：1988-02-01 00:00:00

abstract：:Although the lambda-bearing antibodies represent only 5% of the total mouse serum immunoglobulins, some antigens such as B1355 dextran (alpha (1-3)Dex), the 4-hydroxy-3-nitrophenyl acetyl (NP) and 2,4-dinitro or 2,4,6-trinitrophenyl (DNP/TNP) antigens can induce lambda-positive immune responses. In contrast to the lam...

journal_title：Molecular immunology

authors： Sanchez P,Juy D,Cazenave PA

更新日期：1988-02-01 00:00:00

abstract：:Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...

journal_title：Molecular immunology

authors： Yarmush DM,Murphy RM,Colton CK,Fisch M,Yarmush ML

更新日期：1988-01-01 00:00:00

abstract：:The major product of digestion of bovine IgG2a(A1) with immobilized pepsin is F(ab')2 while similar treatment of IgG2a(A2) yields Fab, Fc and other products. It is postulated that structural differences in the hinge region, including the absence of the most N-terminal disulfide bridge in IgG2a(A2) and a displacement o...

journal_title：Molecular immunology

authors： Heyermann H,Butler JE

更新日期：1987-12-01 00:00:00

abstract：:Four cell lines producing monoclonal antibodies were obtained by fusion of NS1 myeloma cells with splenocytes of BALB/C mice immunized with only 1 microgram of each staphylococcal enterotoxin A, B, C1 and D by a modified technique of intrasplenic boosting. This procedure was considerably more efficient than the more c...

journal_title：Molecular immunology

authors： Lapeyre C,Kaveri SV,Janin F,Strosberg AD

更新日期：1987-12-01 00:00:00

abstract：:Mouse monoclonal antibodies (MAbs) have been prepared against rat T cell blasts. One MAb called MRC OX-40 recognized an antigen that differed from any previously described in that its expression was detected only on T blasts that also expressed the CD4 antigen. The OX-40 MAb did not detect an activation determinant of...

journal_title：Molecular immunology

authors： Paterson DJ,Jefferies WA,Green JR,Brandon MR,Corthesy P,Puklavec M,Williams AF

更新日期：1987-12-01 00:00:00

abstract：:The binding properties of 18 monoclonal antibodies (MAbs) directed against the tobacco mosaic virus (TMV) coat protein were studied with five related tobamoviruses and seven mutant viruses, as well as with the dissociated coat proteins of these variants. Ten of the antibodies bound to both TMV and TMV protein, but the...

journal_title：Molecular immunology

authors： Dore I,Altschuh D,Al Moudallal Z,Van Regenmortel MH

更新日期：1987-12-01 00:00:00

abstract：:Very sensitive assays of IgE are required for determining prevalence of allergic reactions in children. In order to develop a sensitive two-site IRMA two kinds of mAb were produced. Antibodies specific for D epsilon 1 determinants were derived from immunization with a 40 kDa papain Fc fragment. They bound equally nati...

journal_title：Molecular immunology

authors： Anfosso F,Demeure C,Delaage M,Cheballah R,Bellot F,Bourgois A

更新日期：1987-11-01 00:00:00

abstract：:Jackfruit lectin, jacalin, prepared from two batches of jackfruit seeds showed a different specificity in precipitating reaction in Agarose gel with various purified immunoglobulins and secretory components. Jacalin-P, extracted from jackfruit seeds from the Philippines, reacts only with serum IgA and secretory IgA of...

journal_title：Molecular immunology

authors： Kondoh H,Kobayashi K,Hagiwara K

更新日期：1987-11-01 00:00:00

abstract：:The human monocyte-like cell line, U-937, was shown to synthesize and secrete C3 by hemolytic assays, radioimmunoassays and metabolic labeling experiments. The daily synthesis of antigenic C3 by unstimulated U-937 cells was low (about 3 ng/10(6) cells/24 hr) over a 3 day period. Induction of the cells to differentiate...

journal_title：Molecular immunology

authors： Minta JO,Isenman DE

更新日期：1987-10-01 00:00:00

abstract：:In extension of the hypothesis that an amphipathic alpha helix of Ii (Phe146-Val164) bound to the foreign antigen-presenting site (desetope) of class II MHC molecules through hydrophobic amino acid residues (Phe146, Leu150, Leu153, Met157, Ile160, Val164) which were present in an axial strip along one side of the Ii h...

journal_title：Molecular immunology

authors： Stille CJ,Thomas LJ,Reyes VE,Humphreys RE

更新日期：1987-10-01 00:00:00

abstract：:The kinetics of the cryoprecipitation of two monoclonal IgG and two monoclonal IgM cryoimmunoglobulins, two IgM/IgG mixed cryoglobulins and a series of cold soluble monoclonal IgG and IgM immunoglobulins in the presence of polyethylene glycol have been compared by time dependent turbidity measurements. The effects of ...

journal_title：Molecular immunology

authors： Lawson EQ,Brandau DT,Trautman PA,Aziz SE,Middaugh CR

更新日期：1987-09-01 00:00:00

abstract：:Since secretion of IgE antibodies is known to be blocked by tunicamycin, the first aim of the present study was to determine at which step of glycosylation or processing secretion was restored. For this purpose, murine hybridoma cells secreting an anti beta-lactoglobulin IgE were incubated either in the presence of in...

journal_title：Molecular immunology

authors： Granato DA,Neeser JR

更新日期：1987-08-01 00:00:00

abstract：:In a previous study we isolated a series of rat monoclonal antibodies to the human leukocyte common (LC) antigen and demonstrated that synergistic complement lysis was possible between IgG2b antibodies which recognised different epitopes. In this report we have examined the mechanisms that were involved in synergistic...

journal_title：Molecular immunology

authors： Bindon CI,Hale G,Hughes-Jones N,Gorick B,Waldmann H

更新日期：1987-06-01 00:00:00

abstract：:Binding of the catecholamine beta-adrenergic antagonist, l-alprenolol, by the IgGl anti-alprenolol monoclonal antibody 37A4 was examined using the radioligand 3H-dihydroalprenolol as an extrinsic signal and the increase in antibody fluorescence upon l-alprenolol binding as intrinsic signal. Equilibrium binding studies...

journal_title：Molecular immunology

authors： Hoebeke J,Engelborghs Y,Chamat S,Strosberg AD

更新日期：1987-06-01 00:00:00

abstract：:We examined the expression of T15 idiotopes (Id) on phosphorylcholine (PC)-binding monoclonal immunoglobulins and defined the structural correlates of these Id. The seven monoclonal anti-Id antibodies used as probes recognize distinct determinants that range from the antigen binding site to the CH1 domain on TEPC15. C...

journal_title：Molecular immunology

authors： Strickland FM,Gleason JT,Cerny J

更新日期：1987-06-01 00:00:00

abstract：:Monoclonal antibodies were prepared against synthetic peptides synthetized on the basis of nucleotide sequence from cDNA and genomic clones encoding a class I antigen expressed by the rabbit RL-5 cell line. Using a peptide corresponding to positions 61-73 of the N domain, we were able to obtain an hybridoma producing ...

journal_title：Molecular immunology

authors： Le Guern A,Wetterskog D,Marche PN,Kindt TJ

更新日期：1987-05-01 00:00:00

abstract：:The ability of bovine IgG preparations to bind to the various distinct human leukocyte Fc gamma receptors was studied. In experiments using intact cells and isolated Fc gamma receptors, it was demonstrated that bovine IgG can bind to Fc gamma receptors of four human cell types but not to Fc gamma receptors of human ne...

journal_title：Molecular immunology

authors： Kulczycki A Jr

更新日期：1987-03-01 00:00:00

abstract：:Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...

journal_title：Molecular immunology

authors： Bailly P,Chevaleyre J,Sondag D,François-Gérard C,Piquet Y,Vezon G,Cartron JP

更新日期：1987-02-01 00:00:00

abstract：:Twenty-seven murine monoclonal anti-hen egg-white lysozyme (HEL) antibodies were tested with a panel of nine antigens; eight avian lysozymes and human lysozyme. The antibodies were arranged into 10 groups based on their antigen specificity and cross-reactivity. Antigenic determinants recognized by each group of antibo...

journal_title：Molecular immunology

authors： Harper M,Lema F,Boulot G,Poljak RJ

更新日期：1987-02-01 00:00:00

abstract：:Chicken and turkey beta 2-m were isolated from citrated plasma in sequential use of three chromatographic steps: affinity chromatography, gel filtration chromatography and anion-exchange chromatography. The purified protein was identified as beta 2-m by reaction with a beta 2-m specific monoclonal antibody and by the ...

journal_title：Molecular immunology

authors： Skjødt K,Welinder KG,Crone M,Verland S,Salomonsen J,Simonsen M

更新日期：1986-12-01 00:00:00

abstract：:The functional importance of various cell membrane bound molecules was studied and compared in the NK cytotoxicity and CTL activity. LFA-1 and CD2 participate in both killing functions, while CD3 and CD8/CD4 as well as MHC class I molecules are involved only in CTL activity. Nevertheless CD2- and beta 2-microglobulin ...

journal_title：Molecular immunology

authors： Petrányi GG,Pócsik E,Kotlán B,Görög G,Benczur M

更新日期：1986-11-01 00:00:00

abstract：:The generation and cell surface expression of IL-2 receptors was monitored by: (i) an ELISA that permits quantitative determination of detergent-solubilized or soluble IL-2 receptors; and (ii) detection of the binding of 125I-labelled recombinant IL-2 and of anti-IL-2 receptor antibodies to receptor bearing cells. Upo...

journal_title：Molecular immunology

authors： Diamantstein T,Osawa H,Mouzaki A,Josimovic-Alasevic O

更新日期：1986-11-01 00:00:00

abstract：:The binding specificity of rat alveolar macrophages (AM phi) for sheep erythrocytes (E) coated with gangliosides GM1, GM2, GM3, GD1a, GD1b or GT1b was analyzed in a rosette assay by studying the inhibitory effect of gangliosides, various carbohydrates, IgG, C3b-like C3, and fibronectin in this assay. The uptake of gan...

journal_title：Molecular immunology

authors： Förster O,Boltz-Nitulescu G,Holzinger C,Wiltschke C,Riedl M,Ortel B,Fellinger A,Bernheimer H

更新日期：1986-11-01 00:00:00